期刊
TOXICOLOGY IN VITRO
卷 72, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2021.105076
关键词
Paraquat; Inflammatory microglia; Neuroinflammatory responses; NF-kappa B; PDTC
类别
资金
- Ningxia Natural Science Foundation [2020AAC02018]
- Special talents start-up funding of Ningxia Medical University [XT2019013]
Paraquat can activate microglia to induce inflammation responses, which in turn have secondary inflammatory effects on neurons, mainly by activating the NF-κB pathway to increase the release of TNF-α and IL-6.
Paraquat (PQ), a non-selective contact herbicide, has been generally accepted as one of the environmental neurotoxicants. Despite the direct evidence that PQ could induce inflammation responses in microglia, little is known about the effects of the inflammatory microglia on neurons. Thus in the present study, mouse primary cortical neurons and PC12 cells, widely-used in vitro neuron models for neurotoxicity research were applied to investigate the neuroinflammatory effects of PQ-activated microglia on neurons. We observed that the secretion levels of TNF-alpha and IL-6 in PC12 cells were markedly increased upon treatment with the supernatants of inflammatory BV2 microglia, and NF-kappa B p65 protein expression was also elevated. Specific inhibition of NF-kappa B by PDTC dramatically attenuated the increase of TNF-alpha and IL-6 release. These results suggested that PQ-induced inflammatory microglia exerts secondary inflammatory effects on neurons through activation of NF-kappa B pathway.
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