Sandwich-type electrochemical immunosensor for CEA detection using magnetic hollow Ni/C@SiO2 nanomatrix and boronic acid functionalized CPS@PANI@Au probe

A sandwich-type electrochemical immunosensor was developed for the detection of CEA where hollow magnetic silica coated nickel/carbon (Ni/C@SiO2) nanocomposites was used as an immobilized carrier and gold nanoparticle-coated PANI microsphere (CPS@PANI@Au) as electrochemical transducer. Magnetic assembly of Ni/C@SiO2 nanocomposites allow easy separation and assembly which eliminates the further modification process. In addition, the prepared CPS@PANI@Au possess good biocompatibility, and electrical conductivity. The fabricated immunosensor show excellent sensing performance including a wide dynamic range from 0.006 to 12.00 ng mL(-1) and low detection limit at 1.56 pg mL 1. Moreover, this sensor shows superior selectivity with excellent reliability and reproducibility which might lead into easier implementation in clinical setting.

Automated summary

A sandwich-type electrochemical immunosensor was developed for the detection of CEA, with excellent sensing performance and low detection limit. The magnetic assembly of nanocomposites simplifies further modification process, improving the reliability and reproducibility of the sensor.