期刊
TALANTA
卷 225, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.talanta.2020.122019
关键词
Single cells; Inkjet printing; Sodium alginate; Cell analysis; Enzyme kinetic
资金
- National Natural Science Foundation of China (NSFC) [21804045]
- Natural Science Foundation of Fujian Province [2018J05020]
- Fujian Transportation Industry Key Science and Technology Project [2018-MS5-129]
- Promotion Program for Young and Middle-aged Teachers in Science and Technology Research of Huaqiao University [ZQN-PY612]
This study developed a convenient and mask-free approach for single-cell analysis using inkjet printing technology and PDMS microchip, allowing precise control over cell number and real-time monitoring of cell performance attributes.
A convenient, facile, and mask-free approach assay was developed for single-cell study by using a combination of inkjet printing technology and polydimethylsiloxane (PDMS) microchip-assisted processing. The inkjet printing technology resulted in 91% of the single-cell occupancy by individually spraying MCF-7 cells on a hydrophobic substrate and enabled the control over the number of cells with precision by strictly optimizing the printing parameters. Further, the microchip containing a cell chamber and straight channels was attached to the glass slide to explore the real-time performance of the cells. To address the performance attributes, the enzyme kinetics and various parameters of the post-printed MCF-7 cells, such as the levels of cell viability, reactive oxygen species (ROS), cell apoptosis, and proliferation, are monitored in real-time. Interestingly, high activity and proliferation, low level of ROS, and cell apoptosis demonstrated that the developed method provided a new way to the study of single-cell in-depth. Finally, ATP-induced cell proliferation of different cell number were analyzed, and the results would provide another perspective for the diagnosis and medical treatment.
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