期刊
SYSTEMATIC AND APPLIED MICROBIOLOGY
卷 44, 期 3, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.syapm.2021.126204
关键词
Campylobacter vulpis sp. nov.; Polyphasic taxonomic study; Red foxes; Vulpes vulpes
资金
- University of Bologna (Italy) [RFO14]
- United States Department of Agriculture, Agricultural Research Service, CRIS project [203042000230051]
A study identified a novel species of Campylobacter, Campylobacter vulpis sp. nov., through a polyphasic approach, including genetic comparisons and genome sequencing. A specific PCR test based on atpA gene sequences was designed for rapid discrimination from the closely-related C. upsaliensis.
During a sampling of wild red foxes (Vulpes vulpes) for the detection of Epsilonproteobacteria, 14 strains were isolated from the caecal contents of 14 epidemiologically-unrelated animals. A genus-specific PCR indicated that the isolates belonged to the genus Campylobacter. Based on the results of a species-specific PCR, the isolates were initially identified as C. upsaliensis. However, multi-locus sequence typing (MLST) revealed that the isolates were significantly different from the C. upsaliensis present in the MLST database. A polyphasic study, including conventional biochemical and tolerance characteristics, morphology by transmission electron microscopy (TEM), MALDI-TOF analysis, and genetic comparisons based on partial 16S rDNA and atpA gene sequences, was undertaken. Finally, the complete genome sequence of the type strain 251/13(T) and the draft genome sequences of the other isolates were determined. Average nucleotide identity, average amino acid identity and in silico DNA-DNA hybridization analyses confirmed that the isolates represent a novel taxon for which the name Campylobacter vulpis sp. nov. is proposed, with isolate 251/13(T) (=CCUG 70587(T) = LMG 30110(T)) as the type strain. In order to allow a rapid discrimination of C. vulpis from the closely-related C. upsaliensis, a specific PCR test was designed, based on atpA gene sequences. (C) 2021 Elsevier GmbH. All rights reserved.
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