期刊
STEM CELL RESEARCH
卷 52, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scr.2021.102206
关键词
-
资金
- Federal Ministry of Education and Research (BMBF) [iCARE 01EK1601A (-F)]
This article describes a transgenic hiPSC line with dual reporter genes, which can be used to monitor engraftment, calcium fluctuations, and coupling of iPSC derivatives such as cardiomyocytes.
Transgenic hiPSC lines carrying reporter genes represent valuable tools for functional characterization of iPSC derivatives, disease modelling and clinical evaluation of cell therapies. Here, the hiPSC line 'Phoenix' (Haase et al., 2017) was genetically engineered using TALEN-based integration of the calcium sensor GCaMP6f and RedStar(nuc) reporter into the AAVS1 site. Characterization of undifferentiated cells and functional investigation of hiPSC-derived cardiomyocytes-containing BCTs showed a strong intracellular calcium transient-dependent GCaMP6f and eminent RedStar(nuc) signal. Therefore, our dual reporter line provides an excellent tool to facilitate monitoring of engraftment, calcium fluctuations and coupling of iPSC derivatives such as cardiomyocytes in vitro and in vivo in animal models.
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