期刊
STEM CELL RESEARCH
卷 53, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scr.2021.102389
关键词
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资金
- Science Foundation Ireland [13/IA/1787]
- NUI Galway
- Irish Government's Programme for Research in Third Level Institutions, Cycles 4 and 5, National Development Plan 2007-2013
- Irish Research Council
- National Children's Research Centre (NCRC)
- Galway University Foundation
- China Scholarship Council
- FutureNeuro [16/RC/3948]
- Science Foundation Ireland (SFI) [13/IA/1787] Funding Source: Science Foundation Ireland (SFI)
Three human induced pluripotent stem cell (hiPSC) lines were generated from a healthy 7-year-old boy using non-integrational Sendai reprogramming method expressing specific genes. The stem cells were characterized through morphology, immunofluorescence staining, RT-qPCR, and showed differentiation potential to all three germ layers with lineage verification and normal molecular karyotyping.
We report the generation of three human induced pluripotent stem cell (hiPSC) lines (NUIGi047-A, NUIGi047-B, NUIGi047-C) from a healthy 7-year-old boy using non-integrational Sendai re-programming method expressing OCT4, SOX2, KLF4 and C-MYC. Stem cell characterization was confirmed through morphology, immunofluorescence staining and RT-qPCR. Differentiation potential in vitro was demonstrated to all three germ layers with STR lineage verification and normal molecular karyotyping through the process of re-programming.
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