Spectrofluorimetric analysis of fingolimod via complex formation with eosin Y in its pure form, pharmaceutical preparation and biological samples

This work discuss a simple, rapid, accurate, precise, sensitive, validated and effective cost spectrofluorometric method. The technique was applied for the analysis of fingolimod hydrochloride (FIN) in pure form, capsules, human plasma and urine samples. Formation of binary complex between the suggested amino group of (FIN) with Eosin Y (EOY) is the principle of its determination. FIN was determined spectrofluorimetrically by measuring its quenching effect on the EOY native fluorescence at 575 nm after excitation at 525 nm. The fluorescence-concentration linearity was 0.1-1.0 mg mL(-1). The suggested spectrofluorimetric results have been certified according to ICH regulations and were applied for analysis of FIN in capsules, human plasma and urine samples. The validated results were accepted compared to reference method. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

This study introduces a simple and effective spectrofluorometric method for the analysis of fingolimod hydrochloride (FIN). The validated technique shows the ability to accurately determine the concentration of FIN in various samples, meeting the requirements of relevant regulations.