期刊
SMALL
卷 17, 期 23, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202100797
关键词
blood plasma separation; diamagnetophoresis; microfluidics
类别
资金
- Samsung Research Funding Center for Future Research [SRFC-IT1602-02]
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [NRF-2019R1C1C1006124]
- National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2018R1A6A3A11043793]
- UNIST [1.200082.01, 1.200094.01]
- National Research Foundation of Korea [5199990614448] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A hemolysis-free and highly efficient plasma separation platform has been developed using enhanced diamagnetic repulsion of blood cells. By supplementing blood with superparamagnetic iron oxide nanoparticles, complete removal of blood cells from blood plasma is achieved, allowing for successful collection of plasma without losing plasma proteins, platelets, or exosomes.
A hemolysis-free and highly efficient plasma separation platform enabled by enhanced diamagnetic repulsion of blood cells in undiluted whole blood is reported. Complete removal of blood cells from blood plasma is achieved by supplementing blood with superparamagnetic iron oxide nanoparticles (SPIONs), which turns the blood plasma into a paramagnetic condition, and thus, all blood cells are repelled by magnets. The blood plasma is successfully collected from 4 mL of blood at flow rates up to 100 mu L min(-1) without losing plasma proteins, platelets, or exosomes with 83.3 +/- 1.64% of plasma volume recovery, which is superior over the conventional microfluidic methods. The theoretical model elucidates the diamagnetic repulsion of blood cells considering hematocrit-dependent viscosity, which allows to determine a range of optimal flow rates to harvest platelet-rich plasma and platelet-free plasma. For clinical validations, it is demonstrated that the method enables the greater recovery of bacterial DNA from the infected blood than centrifugation and the immunoassay in whole blood without prior plasma separation.
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