期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 334, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.129636
关键词
MIP sensor; Serum; Urine; Simultaneous analysis; FIA
资金
- National Research Council of Thailand (NRCT) [NRCT5RSA6302302]
- Faculty of Science, Ubon Ratchathani University
- Center of Excellence for Innovation in Chemistry (PERCHCIC)
- Ministry of Higher Education, Science, Research and Innovation
- National Research Council of Thailand [IRN/502/2563]
A novel amperometric dual-imprinted sensor was developed for simultaneous determination of creatinine and 8-hydroxy-2'-deoxyguanosine in human urine and serum. The sensor utilized multiple-pulse amperometric detection and two specific nanocomposites to selectively detect the compounds. The proposed method showed promising results for the accurate quantification of Cre and 8-OHdG in biological samples.
We present a novel amperometric dual-imprinted sensor for simultaneous determination of creatinine (Cre) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in human urine and serum. The sensor used multiple-pulse amperometric detection in flow injection analysis (MPA-FIA). Copper oxide nanoparticles were coated with the Cre molecularly-imprinted polymer (CuO@MIP), using methacrylic acid as the functional monomer and N, N'-(1,2-dihydroxyethylene) bis-acrylamide as cross-linker. For 8-OHdG sensing, we embedded platinum nanoparticles in reduced graphene oxide and then coated it with guanosine poly-dopamine MIP (PtNPs-rGO@MIP). A carbon paste electrode (CPE) was then formed containing both nanocomposites to give the dual MIP sensor (CuO@MIP and PtNPs-rGO@MIP/CPE). We developed a dual-potential waveform as a function of time, with (Edet.1) (+0.4 V/150 ms) to determine Cre selectively and E-det.2 (+0.6 V/250 ms) to analyze both compounds simultaneously (Cre and 8-OHdG). Subtracting the two signals at 0.6 V and 0.4 V (using a correction factor), respectively, from each other allowed for quantifying 8-OHdG without interference from Cre. The MIP sensor has a linear range of 0.5-150 mu M for creatinine and 0.005-50 mu M for 8-OHdG, with limits of detection in nano-molar level. The proposed method is successfully applied for the simultaneous determination of Cre and 8-OHdG in urine and serum samples.
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