期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 333, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.129556
关键词
Ratiometric electrochemistry; Competitive host-guest recognition; DNAzyme; miRNA-21; Biosensor
资金
- National Natural Science Foundation of China [21703199, 21773203]
- Lvyangjinfeng Talent Program of Yangzhou - Priority Academic Program Development of Jiangsu Higher Education Institutions
A novel ratiometric electrochemical biosensor was developed for sensitive detection of miRNA-21 by combining DNAzyme-cleavage cycling with competitive host-guest inclusion. The biosensor showed wide concentration range, low detection limit, high sensitivity, good selectivity, satisfactory reproducibility, and stability under optimal conditions. This sensing strategy may offer a new approach in molecular diagnosis and bioanalysis.
A novel ratiometric electrochemical biosensor was designed for sensitive miRNA-21 detection by combining Mg2+ - dependent DNAzyme-cleavage cycling with competitive host-guest inclusion. The dual ferrocene-labeled hairpin DNA (2Fc-DNA)-assembled triple-helix magnetic probe (THMP) and methylene blue (MB)-immobilized reduced graphene oxide/beta-cyclodextrin nanocomposites-modified glassy carbon electrode (RGO/beta-CD/GCE) were served as target recognition and signal transformation units, respectively. Upon the hybridization reaction between target miRNA-21 and two auxiliary probes, the active DNAzyme was liberated from the caged structure, which bound with 2Fc-DNA to catalyze its cleavage in the presence of Mg2+, and triggered continuous cleavage reaction to obtain a large number of Fc-labeled DNA fragments. After magnetic separation, Fc-labeled DNA fragments could replace MB by the competitive host-guest recognition reaction and accumulate on the electrode surface, resulting in the gradually enhanced Fc and weakened MB signals, respectively. Under the optimal conditions, the constructed biosensor with ratiometric detection mode showed wide concentration range (10 fM-0.1 nM), low detection limit (1.8 fM), high sensitivity, and good selectivity, as well as satisfactory reproducibility and stability. And the developed sensing strategy may provide a new pathway in molecular diagnosis and bioanalysis.
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