期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 335, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.129698
关键词
Photosensitizer; Aggregation-induced emission; Lysosome imaging; Photodynamic therapy; Tumor
资金
- National Natural Science Foundation of China Project [81901811, 81801832]
- Postdoctoral Science Foundation of China [2019T120491, 2019M625046]
- Scientific Research Fund of Zhejiang Provincial Education Department [Y201940895]
- Ningbo Public Welfare Science and Technology Project [2019C50072, 202002N3157]
- Natural Science Foundation of Ningbo [2018A610294, 2019A610207]
- Fundamental Research Funds for the Provincial Universities of Zhejiang [SJLY2021008]
- K.C. Wong Magna Fund from Ningbo University
A lysosome-targeting photosensitizer MP-TPEDCH with excellent photostability and lysosome targeting specificity has been designed in this work, showing significantly enhanced antitumor effect under NIR irradiation.
Lysosome is an indispensable organelle and its dysfunction often leads to some neurodegenerative diseases. Traditional fluorescent lysosome probes usually have conjugate structures and the pi-pi stacking can lead to aggregation-caused quenching (ACQ), which limits its application in bioimaging. In this work, we have designed an AIEgen-based photosensitizer MP-TPEDCH by introducing a lysosome-targeting moiety of 4-(2-chloroethyl) morpholine (MP) to a fluorescent AIE skeleton TPEDCH. The optimized structure calculated by density functional theory (DFT) reveals it has a propeller-shaped conformation, which severely inhibits the intermolecular pi-pi stacking interaction and therefore results in fluorescence quenching in the isolate state. Moreover, it has a broad emission wavelength up to 850 nm and exhibits typical AIE features. The electronic clouds on highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) are effectively separated, suggesting MP-TPEDCH had a small singlet-triplet energy gap. Encouragingly, it has been demonstrated that MP-TPEDCH showed high photostability, excellent lysosome targeting specificity, well ROS generation ability and dramatically enhanced antitumor effect under NIR irradiation. The proposed probe not only expands the family of lysosome imaging agents but also provides a new strategy for preparing effective photosensitizers in cancer treatment.
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