期刊
SENSORS
卷 21, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/s21062035
关键词
surface plasmon resonance; plasmonic sensor; temperature control; localized heating
资金
- European Regional Development Fund, via the Interreg V France-Wallonie-Vlaanderen programme, under the Biosens project
- University of Lille
Surface plasmon resonance (SPR) sensing is a high-sensitivity, label-free, real-time detection technique used for studying biomolecular interactions by measuring the optical refractive index of the medium. To ensure accuracy, measurements should be conducted in a temperature-controlled environment, with the proposal of using a metallic film as a heater.
Surface plasmon resonance (SPR) sensing is a well-established high-sensitivity, label-free and real-time detection technique for biomolecular interaction study. Its primary working principle consists of the measurement of the optical refractive index of the medium that is in close vicinity of the sensor surface. Bio-functionalization techniques allow biomolecular events to be located in such a way. Since optical refractive indices of any medium varies with the temperature, the place where the measurement takes place shall be within a temperature-controlled environment in order to ensure any temperature fluctuation is interpreted as a biomolecular event. Since the SPR measurement probes the sensed medium within the penetration depth of the plasmonic wave, which is less or in the order of 1 mu m, we propose to use the metallic film constituting the detection surface as a localized heater aiming at controlling finely and quickly the temperature of the sensed medium. The Joule heating principle is then used and the modeling of the heater is reported as well as its validation by thermal IR imaging. Using water as a demonstration medium, SPR measurement results at different temperatures are successfully compared to the theoretical optical refractive index of water versus temperature.
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