Fatty acid profiles of estuarine macroalgae are biomarkers of anthropogenic pressures: Development and application of a multivariate pressure index

Transitional ecosystems are among the most degraded ecosystems worldwide, with several groups of organisms investigated for their reliability as biological indicators of human-driven disturbances. Recently non-traditional biochemical biomarkers such as an individual's fatty acids profile have been identified as promising tools for assessing contaminant exposure. In this work, two abundant Atlantic benthic macroalgae (Ulva lactuca and Fucus vesiculosus species) were surveyed in three mudflat areas of the highly urbanized Tejo estuary, with increasing anthropogenic disturbance degrees (Alcochete, Rosario and Seixal mudflats, increasing in contamination by this order) and their fatty acids evaluated as potential biomarkers for exposure to contaminants known to have toxic effects on biota. In terms of contamination the metal pollution index of all the compartments analysed (sediment bioavailable and total metal concentrations and thallus metal concentrations) revealed the same tendencies with lower contamination levels in Alcochete, intermediate in Rosario and high contamination levels in Seixal. In the thallus of U. lactuca thallus could be observed a strong decrease in C18-fatty acids along the contamination gradient, likely due to lipid peroxidation from metal-generated reactive oxygen species. Nevertheless, an increase in stearic and hexadecatrienoic acids in the thallus from the most contaminated site

Automated summary

Transitional ecosystems, which are highly degraded worldwide, have been investigated for potential biological indicators of human-driven disturbances. Recent studies have shown that non-traditional biochemical biomarkers, such as an individual's fatty acids profile, can be promising tools for assessing contaminant exposure. In this specific study, two abundant Atlantic benthic macroalgae were surveyed in the highly urbanized Tejo estuary, with their fatty acids evaluated as potential biomarkers for exposure to contaminants. The results suggest a correlation between contamination levels and the fatty acid profiles of the macroalgae in different areas of the estuary.