期刊
RNA BIOLOGY
卷 18, 期 12, 页码 2203-2217出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2021.1917215
关键词
Type VI Crispr-Cas (Cas13); engineered soybean ascorbate peroxidase APEX2; proximity-dependent biotinylation; RNA proximity labelling (RPL); RNA proximal proteins; RNA-centric method; RRNA– proteininteractions; poly(A) tail; RNA binding proteins (RBPs); U1 snRNA RBPS; noncoding RNAs (ncRNAs)
资金
- Cedars-Sinai Medical Center [Cancer Biology Program Discovery Fund] [213433]
- National Cancer Institute [K99/R00-CA184415, R01-CA207456]
- Ovarian Cancer Research Alliance [Ann and Sol Schreiber Mentored Investigator Award] [458799]
RNA molecules, including mRNAs encoding proteins and noncoding transcripts, function with the help of RNA binding proteins (RBPs). RNA proximity labelling (RPL), a method utilizing proximity-based biotinylation, has been developed to rapidly identify target RNA binding proteins in native cellular contexts. This technique has the potential to uncover novel RNA-protein interactions important for health and disease.
RNA molecules function as messenger RNAs (mRNAs) that encode proteins and noncoding transcripts that serve as adaptor molecules, structural components, and regulators of genome organization and gene expression. Their function and regulation are largely mediated by RNA binding proteins (RBPs). Here we present RNA proximity labelling (RPL), an RNA-centric method comprising the endonuclease-deficient Type VI CRISPR-Cas protein dCas13b fused to engineered ascorbate peroxidase APEX2. RPL discovers target RNA proximal proteins in vivo via proximity-based biotinylation. RPL applied to U1 identified proteins involved in both U1 canonical and noncanonical functions. Profiling of poly(A) tail proximal proteins uncovered expected categories of RBPs and provided additional evidence for 5MODIFIER LETTER PRIME-3MODIFIER LETTER PRIME proximity and unexplored subcellular localizations of poly(A)(+) RNA. Our results suggest that RPL allows rapid identification of target RNA binding proteins in native cellular contexts, and is expected to pave the way for discovery of novel RNA-protein interactions important for health and disease.
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