期刊
RADIOTHERAPY AND ONCOLOGY
卷 158, 期 -, 页码 7-12出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.radonc.2021.02.003
关键词
Electron flash effect; Oxygen depletion; Normal tissue toxicity; Zebrafish embryo
资金
- European Union (ERC Advanced Grant ZfBrainReg)
- European Union [GINOP-2.3.6-15-2015-00001]
- European Regional Development Fund
- European Union's Horizon 2020 research and innovation programme [871124]
The study demonstrated that the zebrafish embryo model is suitable for investigating the radiobiological response of high dose rate irradiation. It also highlighted the importance of pulse dose rate and partial oxygen pressure (pO(2)) as relevant parameters during radiation.
Background and purpose: In consequence of a previous study, where no protecting proton Flash effect was found for zebrafish embryos, potential reasons and requirements for inducing a Flash effect should be investigated with higher pulse dose rate and partial oxygen pressure (pO(2)) as relevant parameters. Materials and methods: The experiments were performed at the research electron accelerator ELBE, whose variable pulse structure enables dose delivery as electron Flash and quasi-continuously (reference irradiation). Zebrafish embryos were irradiated with similar to 26 Gy either continuously at a dose rate of similar to 6.7 Gy/min (reference) or by 1441 electron pulses within 111 mu s at a pulse dose rate of 10(9) Gy/s and a mean dose rate of 10(5)Gy/s, respectively. Using the OxyLite system to measure the pO(2) a low- (pO(2) <= 5 mmHg) and a high-pO(2) group were defined on basis of the oxygen depletion kinetics in sealed embryo samples. Results: A protective Flash effect was seen for most endpoints ranging from 4 % less reduction in embryo length to about 20-25% less embryos with spinal curvature and pericardial edema, relative to reference irradiation. The reduction of pO(2) below atmospheric levels (148 mmHg) resulted in higher protection, which was however more pronounced in the low-pO(2) group. Conclusion: The Flash experiment at ELBE showed that the zebrafish embryo model is appropriate for studying the radiobiological response of high dose rate irradiation. The applied high pulse dose rate was confirmed as important beam parameter as well as the pivotal role of pO2 during irradiation. (C) 2021 Elsevier B.V. All rights reserved.
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