Experimental evidence of the importance of multitrophic structure for species persistence

Ecological theory predicts that species interactions embedded in multitrophic networks shape the opportunities for species to persist. However, the lack of experimental support of this prediction has limited our understanding of how species interactions occurring within and across trophic levels simultaneously regulate the maintenance of biodiversity. Here, we integrate a mathematical approach and detailed experiments in plant-pollinator communities to demonstrate the need to jointly account for species interactions within and across trophic levels when estimating the ability of species to persist. Within the plant trophic level, we show that the persistence probability of plant species increases when introducing the effects of plant-pollinator interactions. Across trophic levels, we show that the persistence probabilities of both plants and pollinators exhibit idiosyncratic changes when experimentally manipulating the multitrophic structure. Importantly, these idiosyncratic effects are not recovered by traditional simulations. Our work provides tractable experimental and theoretical platforms upon which it is possible to investigate the multitrophic factors affecting species persistence in ecological communities.

Automated summary

This study shows the importance of considering species interactions within and across trophic levels when estimating species persistence. By integrating mathematical approaches and experiments, the researchers demonstrated that idiosyncratic changes occur in the persistence probabilities of both plants and pollinators when manipulating the multitrophic structure, highlighting the need for a more comprehensive understanding of species interactions in ecological communities.