期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2022547118
关键词
receptor-like kinase; BAM1/2; xylem patterning; C4; miRNA
资金
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27040206]
- National Natural Science Foundation of China (NSFC) [31671994, 31870250]
- NSFC [31950410534]
- Marie Sklodowska-Curie Grant from the European Union's Horizon 2020 Research and Innovation Programme [896910-GeminiDECODER]
- Shanghai Center for Plant Stress Biology of the Chinese Academy of Sciences
- 100 Talent Program of the Chinese Academy of Sciences
This study demonstrates that the establishment of xylem patterning in Arabidopsis roots is dependent on the opposing gradients of miRNAs and their targets, with BAM1 and BAM2 playing redundant roles in ensuring proper distribution and accumulation of miR165/6-targeted transcripts, ultimately affecting xylem morphology.
Xylem patterning in the root is established through the creation of opposing gradients of miRNAs and their targets, transcripts of the HD-ZIP III family of transcriptions factors, enabled by the cell-to-cell spread of the former. The miRNAs regulating xylem patterning, miR165/6, move through plasmodesmata, but how their trafficking is regulated remains elusive. Here, we describe that simultaneous mutation of the plasma membrane- and plasmodesmata-localized receptor-like kinases (RLKs) BARELY ANY MERISTEM (BAM) 1 and 2 or expression of the geminivirus-encoded BAM1/2-interactor C4 results in higher accumulation and broader distribution of the HD-ZIP III transcripts despite normal total accumulation of miR165/6, and ultimately causes defects in xylem patterning, which depend on the function of the aforementioned miRNA targets. Taken together, our results showthat BAM1 and BAM2 are redundantly required for proper xylem patterning in the Arabidopsis root, by ensuring the proper distribution and accumulation of miR165/6-targeted transcripts.
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