期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2018505118
关键词
bovine; expanded potential stem cell; nuclear transfer
资金
- National Natural Science Foundation of China [31560335, 32070869]
- Key Science and Technology Planning Project of Inner Mongolia Autonomous Region [2020ZD0007]
- Science and Technology Planning Project of Inner Mongolia Autonomous Region [201702045]
- Research Grants Council (RGC) [GRF 17127219]
- University of Hong Kong
The researchers have successfully established stem cells from porcine and human preimplantation embryos using the EPSC approach, enabling cross-species differentiation. Upon studying bovine EPSCs, they found that these stem cells express high levels of pluripotency genes in feeder-free culture and maintain genetic stability in long-term culture.
Embryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of domestic ungulates and large livestock species has been challenging. In contrast to ESCs that are usually established from the blastocyst, mouse expanded potential stem cells (EPSCs) are derived from four-cell and eight cell embryos. We have recently used the EPSC approach and established stem cells from porcine and human preimplantation embryos. EPSCs are molecularly similar across species and have broader developmental potential to generate embryonic and extraembryonic cell lineages. We further explore the EPSC technology for mammalian species refractory to the standard ESC approaches and report here the successful establishment of bovine EPSCs (bEPSCs) from preimplantation embryos of both wild-type and somatic cell nuclear transfer. bEPSCs express high levels of pluripotency genes, propagate robustly in feeder-free culture, and are genetically stable in long-term culture. bEPSCs have enriched transcriptomic features of early preimplantation embryos and differentiate in vitro to cells of the three somatic germ layers and, in chimeras, contribute to both the embryonic (fetal) and extraembryonic cell lineages. Importantly, precise gene editing is efficiently achieved in bEPSCs, and genetically modified bEPSCs can be used as donors in somatic cell nuclear transfer. bEPSCs therefore hold the potential to substantially advance biotechnology and agriculture.
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