4.8 Article

Structure of Gcn1 bound to stalled and colliding 80S ribosomes

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Summary: Translation stalling activates ribosome quality control (RQC) and is linked to the integrated stress response (ISR) through Gcn2, with Hel2 suppressing Gcn2 activation. Both pathways are activated by similar factors causing ribosome stalling, although Hel2 is maximally activated at a lower frequency. Inactivation of one pathway leads to overactivation of the other, suggesting they are both triggered by the same signal. ISR prefers an empty A site while RQC shows no preference, indicating they do not directly compete with each other.

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