期刊
出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2102188118
关键词
PIEZO; mechanosensation; ion channels; Arabidopsis; root
资金
- Swiss National Science Foundation [P300PA_164695, P2LAP3_151727]
- NIH [GM114660]
- China Scholarship Council
- Howard Hughes Medical Institute
- National Heart, Lung, and Blood Institute [R01HL143297]
- Swiss National Science Foundation (SNF) [P2LAP3_151727, P300PA_164695] Funding Source: Swiss National Science Foundation (SNF)
PIEZO1 in Arabidopsis thaliana plays an important role in root mechanotransduction, establishing PIEZOs as physiologically relevant mechanosensitive ion channels across animal and plant kingdoms.
Plant roots adapt to the mechanical constraints of the soil to grow and absorb water and nutrients. As in animal species, mechanosensitive ion channels in plants are proposed to transduce external mechanical forces into biological signals. However, the identity of these plant root ion channels remains unknown. Here, we show that Arabidopsis thaliana PIEZO1 (PZO1) has preserved the function of its animal relatives and acts as an ion channel. We present evidence that plant PIEZO1 is expressed in the columella and lateral root cap cells of the root tip, which are known to experience robust mechanical strain during root growth. Deleting PZO1 from the whole plant significantly reduced the ability of its roots to penetrate denser barriers compared to wild-type plants. pzo1 mutant root tips exhibited diminished calcium transients in response to mechanical stimulation, supporting a role of PZO1 in root mechanotransduction. Finally, a chimeric PZO1 channel that includes the C-terminal half of PZO1 containing the putative pore region was functional and mechanosensitive when expressed in naive mammalian cells. Collectively, our data suggest that Arabidopsis PIEZO1 plays an important role in root mechanotransduction and establish PIEZOs as physiologically relevant mechanosensitive ion channels across animal and plant kingdoms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据