期刊
PLOS ONE
卷 16, 期 5, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0251055
关键词
-
资金
- European Commission [512145, 241883, 681070]
- Flemish Government (IWT TBM7) [IWT 130138]
- Juvenile Diabetes Research Foundation [4-2005-1327, 17-2013-296]
- Wetenschappelijk Fonds Willy Gepts of the UZ Brussel
Organs from donors after controlled circulatory death (DCD III) have lower beta cell numbers compared to those from brain death donors (DBD), but similar purity and insulin content. Shortening warm ischemia time and using specific preservation solutions can prevent the reduction in beta cell yield in DCD III organs.
Organs from donors after controlled circulatory death (DCD III) exhibit a higher risk for graft dysfunction due to an initial period of warm ischemia. This procurement condition can also affect the yield of beta cells in islet isolates from donor pancreases, and hence their use for transplantation. The present study uses data collected and generated by our Beta Cell Bank to compare the number of beta cells in isolates from DCD III (n = 141) with that from donors after brain death (DBD, n = 609), before and after culture, and examines the influence of donor and procurement variables. Beta cell number per DCD III-organ was significantly lower (58 x 10(6) versus 84 x 10(6) beta cells per DBD-organ; p < 0.001) but their purity (24% insulin positive cells) and insulin content (17 mu g / 10(6) beta cells in DCD III-organs versus 19 mu g / 10(6) beta cells in DBD-organs) were similar. Beta cell number correlated negatively with duration of acirculatory warm ischemia time above 10 min; for shorter acirculatory warm ischemia time, DCD III-organs did not exhibit a lower beta cell yield (74 x 10(6) beta cells). Use of Institut Georges Lopez-1 cold preservation solution instead of University of Wisconsin solution or histidine-tryptophan-ketoglutarate also protected against the loss in beta cell yield from DCD III-organs (86 x 10(6) for IGL-1 versus 54 x 10(6) and 65 x 10(6) beta cells respectively, p = 0.042). Multivariate analysis indicates that both limitation of acirculatory warm ischemia time and use of IGL-1 prevent the reduced beta cell yield in islet cell isolates from DCD III-organs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据