期刊
PLANT PHYSIOLOGY AND BIOCHEMISTRY
卷 162, 期 -, 页码 74-85出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2021.02.021
关键词
Glucose-6-phosphate dehydrogenase; Antioxidant; Reduced glutathione; Salt stress; Seed germination; Soybean
资金
- National Natural Science Foundation of China [31972494, 31671595, 31670244]
- Agricultural Biotechnology Research and Application Development Program of Gansu Province [GNSW-2016-23]
- Foundation of Science and Technology of Gansu Province [20JR5RA288]
- Fundamental Research Funds for the Central Universities [lzujbky-2020-sp04]
Research shows that soybean cytosolic G6PD gene GmG6PD7 increases plant salt tolerance by reducing ROS accumulation and regulating ABA metabolism pathways to improve seed germination rate and primary root growth under salt stress.
Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) plays an important role in response to salt stress in plants. However, much less is known about G6PD proteins in soybean (Glycine max L.). Here, we found that a soybean cytosolic G6PD gene, GmG6PD7, was induced by NaCl. We generated Arabidopsis transgenic lines overexpressing GmG6PD7. The seed germination rate and primary root length of Arabidopsis thaliana overexpressing GmG6PD7 under NaCl treatment were enhanced. Salt stress induced an obvious increase of the total and cytosolic G6PD activity and the marked decrease of ROS levels in the transgenic plants. At the same time, over-expressing GmG6PD7 in Arabidopsis affected the glutathione and NADPH level and activated ROS scavengers, suggesting that GmG6PD7 contributes to increase salinity tolerance by decreasing ROS accumulation. What's more, we found GmG6PD7 overexpression led to the up-regulation of abscisic acid (ABA) degradation gene and the down-regulation of ABA synthesis and ABA-responsive genes, which finally reduced ABA content to improve seed germination rate under salinity stress. It was noteworthy that GmG6PD7 can rescue the seed and root phenotype of Arabidopsis cytosolic G6PD mutant (Atg6pd5 and Atg6pd6) under salt stress, suggesting cytosolic G6PD may have a conserved function in soybean and Arabidopsis.
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