期刊
PLANT JOURNAL
卷 107, 期 1, 页码 287-302出版社
WILEY
DOI: 10.1111/tpj.15278
关键词
Arabidopsis thaliana; plant growth; lipid identification; lipid fragments; liquid chromatography mass spectrometry; untargeted lipid analysis; lipidomics
资金
- Australian Government's National Collaborative Research Infrastructure Scheme (NCRIS)
- Australian Commonwealth Government
- University of Melbourne
- Agilent Technologies, Australia
- Australian Research Council Future Fellowship [FT160100276]
- University of Melbourne Botany Foundation
- Australian Research Council [FT160100276] Funding Source: Australian Research Council
Mass spectrometry is the primary analytical tool used in plant lipidomics, but faces challenges due to the complexity of plant lipids. A robust pipeline was established to extract and analyze lipid profiles from different tissues and developmental stages of Arabidopsis thaliana, creating an online lipid map for visualization and exploration.
Mass spectrometry is the predominant analytical tool used in the field of plant lipidomics. However, there are many challenges associated with the mass spectrometric detection and identification of lipids because of the highly complex nature of plant lipids. Studies into lipid biosynthetic pathways, gene functions in lipid metabolism, lipid changes during plant growth and development, and the holistic examination of the role of plant lipids in environmental stress responses are often hindered. Here, we leveraged a robust pipeline that we previously established to extract and analyze lipid profiles of different tissues and developmental stages from the model plant Arabidopsis thaliana. We analyzed seven tissues at several different developmental stages and identified more than 200 lipids from each tissue analyzed. The data were used to create a web-accessible in silico lipid map that has been integrated into an electronic Fluorescent Pictograph (eFP) browser. This in silico library of Arabidopsis lipids allows the visualization and exploration of the distribution and changes of lipid levels across selected developmental stages. Furthermore, it provides information on the characteristic fragments of lipids and adducts observed in the mass spectrometer and their retention times, which can be used for lipid identification. The Arabidopsis tissue lipid map can be accessed at .
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