期刊
PLANT DISEASE
卷 105, 期 5, 页码 1490-1494出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PDIS-09-20-1900-RE
关键词
genetic diversity; Pierce's disease; MLSA; MLST; molecular phylogeny; Vitis vinifera
资金
- SENASICA MEXICO grant 2017-2018
- Consejo Nacional de Ciencia y Tecnologia (CONACyT) [FC 01234]
- CONACyT
X.fastidiosa is a phytopathogenic bacterium affecting various crops in Mexico, with genetically diverse populations found among grapevine samples. Subspecies identification and genotyping revealed differences between northern and central Mexican populations of X.fastidiosa.
Xylella fastidiosa is a xylem-inhabiting phytopathogenic bacterium that affects diverse agriculturally relevant crops. In Mexico, X. fastidiosa has been reported in the states of Baja California, Coahuila, and Queretaro. In order to determine the genetic diversity of this bacterium in Mexico, 408 grapevine samples were collected from the main producing states in Mexico. For X. fastidiosa identification, real-time PCR and three-loci end-point PCR were employed. The genotyping of the subspecies was carried out using multilocus sequence typing and analysis, based on seven housekeeping genes: leuA, petC, malF, cysG, holC, nuoL, and gltT. The resulting sequences were compared with those present in extant databases. The presence of X. fastidiosa subsp. fastidiosa in the states of Baja California (sequence type 1), Coahuila (sequence type 1), and Queretaro was confirmed. The isolates from northern Mexico bear high similarity to grapevine isolates from the United States. However, the isolates from Queretaro showed significant differences with currently known sequences, showing that there is genetic variability among the X. fastidiosa subsp. fastidiosa populations from grapevines in northern and central Mexico.
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