期刊
PLANT BIOTECHNOLOGY JOURNAL
卷 19, 期 9, 页码 1725-1742出版社
WILEY
DOI: 10.1111/pbi.13586
关键词
safflower; linoleic acid; flavonoid; genome; evolution; transcriptome
资金
- National Natural Science Foundation of China [32072123]
- Construction Plan of Hubei Province Science and Technology Basic Conditions Platform [2017BEC014]
- Fund for Key Laboratory Construction of Hubei Province [2018BFC360]
- Major Technological Innovation of Hubei Province of China [2018ABA093]
- Hubei Provincial Natural Science Foundation of China [2019CFB214]
- Start-up Fund of Huazhong Agricultural University (HZAU)
- Bioinformatics Computing Platform of National Key Laboratory of Crop Genetic Improvement, HZAU
Safflower, a popular crop with high linoleic acid and flavonoid content, diverged from artichoke and sunflower millions of years ago. Unique expansions in gene families related to lipid metabolism and flavonoid biosynthesis were found in safflower, with key genes such as FAD2 and CHS exhibiting tandem duplications. This study provides insights into the evolutionary history and molecular basis of fatty acid and flavonoid biosynthesis in safflower.
Safflower (Carthamus tinctorius L.), a member of the Asteraceae, is a popular crop due to its high linoleic acid (LA) and flavonoid (such as hydroxysafflor yellow A) contents. Here, we report the first high-quality genome assembly (contig N50 of 21.23 Mb) for the 12 pseudochromosomes of safflower using single-molecule real-time sequencing, Hi-C mapping technologies and a genetic linkage map. Phylogenomic analysis showed that safflower diverged from artichoke (Cynara cardunculus) and sunflower (Helianthus annuus) approximately 30.7 and 60.5 million years ago, respectively. Comparative genomic analyses revealed that uniquely expanded gene families in safflower were enriched for those predicted to be involved in lipid metabolism and transport and abscisic acid signalling. Notably, the fatty acid desaturase 2 (FAD2) and chalcone synthase (CHS) families, which function in the LA and flavonoid biosynthesis pathways, respectively, were expanded via tandem duplications in safflower. CarFAD2-12 was specifically expressed in seeds and was vital for high-LA content in seeds, while tandemly duplicated CarFAD2 genes were upregulated in ovaries compared to CarFAD2-12, which indicates regulatory divergence of FAD2 in seeds and ovaries. CarCHS1, CarCHS4 and tandem-duplicated CarCHS5 similar to CarCHS6, which were up-regulated compared to other CarCHS members at early stages, contribute to the accumulation of major flavonoids in flowers. In addition, our data reveal multiple alternative splicing events in gene families related to fatty acid and flavonoid biosynthesis. Together, these results provide a high-quality reference genome and evolutionary insights into the molecular basis of fatty acid and flavonoid biosynthesis in safflower.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据