期刊
PHYSICS REPORTS-REVIEW SECTION OF PHYSICS LETTERS
卷 921, 期 -, 页码 1-53出版社
ELSEVIER
DOI: 10.1016/j.physrep.2021.03.003
关键词
Nuclear pore complex; Intrinsically disordered proteins; Nanochannels; Stochastic transport; Crowding; Biomimetic; Multivalency; Diffusion; Molecular modeling; Molecular dynamics
资金
- UK BBSRC [BB/J014567/1]
- EPSRC, United Kingdom [EP/L015277/1, EP/L504889/1]
- NIH, United States of America [R35 GM119755]
- National Science Foundation (US) [1943488]
- Swiss National Science Foundation
- Biozentrum, Switzerland
- Swiss Nanoscience Institute
- Zernike Institute for Advanced Materials (University of Groningen), Netherlands
- Dutch Research Council (NWO), Netherlands
- National Science and Engineering Research Council of Canada (NSERC) [RGPIN-2016-06591]
- Deutsche Forschungsgemeinschaft (DFG), Germany [SFB1129, SPP2191, SMPFv2.0]
- UCL-University of Toronto Strategic Partner Fund, Canada-UK
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1943488] Funding Source: National Science Foundation
Eukaryotic cells are characterized by a nucleus containing the genome, surrounded by a nuclear envelope. They have evolved a molecular nanomachine called the Nuclear Pore Complex (NPC) to facilitate bi-directional transport between the cell nucleus and cytoplasm. The NPC combines high molecular specificity with high throughput and speed, and is robust to molecular noise and structural perturbations.
The hallmark of eukaryotic cells is the nucleus that contains the genome, enclosed by a physical barrier known as the nuclear envelope (NE). On the one hand, this compartmentalization endows the eukaryotic cells with high regulatory complexity and flexibility. On the other hand, it poses a tremendous logistic and energetic problem of transporting millions of molecules per second across the nuclear envelope, to facilitate their biological function in all compartments of the cell. Therefore, eukaryotes have evolved a molecular nanomachine'' known as the Nuclear Pore Complex (NPC). Embedded in the nuclear envelope, NPCs control and regulate all the bi-directional transport between the cell nucleus and the cytoplasm. NPCs combine high molecular specificity of transport with high throughput and speed, and are highly robust with respect to molecular noise and structural perturbations. Remarkably, the functional mechanisms of NPC transport are highly conserved among eukaryotes, from yeast to humans, despite significant differences in the molecular components among various species. The NPC is the largest macromolecular complex in the cell. Yet, despite its significant complexity, it has become clear that its principles of operation can be largely understood based on fundamental physical concepts, as have emerged from a combination of experimental methods of molecular cell biology, biophysics, nanoscience and theoretical and computational modeling. Indeed, many aspects of NPC function can be recapitulated in artificial mimics with a drastically reduced complexity compared to biological pores. We review the current physical understanding of the NPC architecture and function, with the focus on the critical analysis of experimental studies in cells and artificial NPC mimics through the lens of theoretical and computational models. We also discuss the connections between the emerging concepts of NPC operation and other areas of biophysics and bionanotechnology. (C) 2021 Elsevier B.V. All rights reserved.
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