4.6 Article

Exploring the role of neuropeptide S in the regulation of arousal: a functional anatomical study

期刊

BRAIN STRUCTURE & FUNCTION
卷 221, 期 7, 页码 3521-3546

出版社

SPRINGER HEIDELBERG
DOI: 10.1007/s00429-015-1117-5

关键词

Neuropeptide S (NPS); Arousal; Glutamate; Dopamine; Preoptic area; Septum; Sleep; Substance P; Transmitter release; Wakefulness

资金

  1. Swedish Research Council from Karolinska Institutet
  2. Rut&Arvid Wolff foundation
  3. National Hungarian Development Agency [KTIA-NAP-13-1-2013-0001]
  4. Hungarian Brain Research Program [KTIA 13 NAP-A-II/14]
  5. Austrian Science Fund (FWF) [P25375]
  6. Austrian Science Fund (FWF) [P25375, P28146] Funding Source: Austrian Science Fund (FWF)
  7. Austrian Science Fund (FWF) [P 24785, P 28146, P 25375] Funding Source: researchfish

向作者/读者索取更多资源

Neuropeptide S (NPS) is a regulatory peptide expressed by limited number of neurons in the brainstem. The simultaneous anxiolytic and arousal-promoting effect of NPS suggests an involvement in mood control and vigilance, making the NPS-NPS receptor system an interesting potential drug target. Here we examined, in detail, the distribution of NPS-immunoreactive (IR) fiber arborizations in brain regions of rat known to be involved in the regulation of sleep and arousal. Such nerve terminals were frequently apposed to GABAergic/galaninergic neurons in the ventro-lateral preoptic area (VLPO) and to tyrosine hydroxylase-IR neurons in all hypothalamic/thalamic dopamine cell groups. Then we applied the single platform-on-water (mainly REM) sleep deprivation method to study the functional role of NPS in the regulation of arousal. Of the three pontine NPS cell clusters, the NPS transcript levels were increased only in the peri-coerulear group in sleep-deprived animals, but not in stress controls. The density of NPS-IR fibers was significantly decreased in the median preoptic nucleus-VLPO region after the sleep deprivation, while radioimmunoassay and mass spectrometry measurements showed a parallel increase of NPS in the anterior hypothalamus. The expression of the NPS receptor was, however, not altered in the VLPO-region. The present results suggest a selective activation of one of the three NPS-expressing neuron clusters as well as release of NPS in distinct forebrain regions after sleep deprivation. Taken together, our results emphasize a role of the peri-coerulear cluster in the modulation of arousal, and the importance of preoptic area for the action of NPS on arousal and sleep.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据