期刊
NUCLEIC ACIDS RESEARCH
卷 49, 期 8, 页码 4325-4337出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab247
关键词
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资金
- Minerva ARCHES award
- Israel Science Foundation [2673/17, 961/19]
A-to-I RNA editing, mediated by ADAR enzymes, increases transcript and protein diversity. Studying zebrafish editing sites revealed high editing levels in early embryos and reproductive organs, providing insights into editing roles during development and maturity.
A-to-I RNA editing is a common post transcriptional mechanism, mediated by the Adenosine deaminase that acts on RNA (ADAR) enzymes, that increases transcript and protein diversity. The study of RNA editing is limited by the absence of editing maps for most model organisms, hindering the understanding of its impact on various physiological conditions. Here, we mapped the vertebrate developmental landscape of A-to-I RNA editing, and generated the first comprehensive atlas of editing sites in zebrafish. Tens of thousands unique editing events and 149 coding sites were identified with high-accuracy. Some of these edited sites are conserved between zebrafish and humans. Sequence analysis of RNA over seven developmental stages revealed high levels of editing activity in early stages of embryogenesis, when embryos rely on maternal mRNAs and proteins. In contrast to the other organisms studied so far, the highest levels of editing were detected in the zebrafish ovary and testes. This resource can serve as the basis for understanding of the role of editing during zebrafish development and maturity.
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