4.8 Article

A conserved BAH module within mammalian BAHD1 connects H3K27me3 to Polycomb gene silencing

期刊

NUCLEIC ACIDS RESEARCH
卷 49, 期 8, 页码 4441-4455

出版社

OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab210

关键词

-

资金

  1. National Institutes of Health [R01-CA215284, R01CA218600]
  2. Gilead Sciences Research Scholars Program in haematology/oncology
  3. When Everyone Survives (WES) Leukemia Research Foundation
  4. UCRF Stimulus Initiative Grant of UNC Lineberger Comprehensive Cancer Center
  5. University of Arkansas for Medical Sciences Proteomics Core Facility
  6. IDeA National Resource for Proteomics
  7. Translational Research Institute through the National Center for Advancing Translational Sciences of the National Institutes of Health
  8. Center for Translational Pediatric Research Bioinformatics Core Resource [TL1TR003109, P20GM121293, P20GM103625, P20GM103429, S10OD018445, UL1TR003107, R01CA236209]
  9. UNC Lineberger Comprehensive Cancer Center Core Support Grant [P30-CA016086]

向作者/读者索取更多资源

Trimethylation of histone H3 lysine 27 (H3K27me3) plays a crucial role in gene silencing and imprinting, (epi)genome organization, and organismal development. This study reveals that the Bromo Adjacent Homology (BAH) domain within BAH domain-containing protein 1 (BAHD1) is essential for the targeting of BAHD1 to chromatin and optimal repression of H3K27me3-demarcated genes in mammalian cells. Disruption of the direct interaction between BAHD1(BAH) and H3K27me3 leads to chromatin remodeling and increased histone acetylation at Polycomb gene targets, affecting embryonic development.
Trimethylation of histone H3 lysine 27 (H3K27me3) is important for gene silencing and imprinting, (epi)genome organization and organismal development. In a prevalent model, the functional readout of H3K27me3 in mammalian cells is achieved through the H3K27me3-recognizing chromodomain harbored within the chromobox (CBX) component of canonical Polycomb repressive complex 1 (cPRC1), which induces chromatin compaction and gene repression. Here, we report that binding of H3K27me3 by a Bromo Adjacent Homology (BAH) domain harbored within BAH domain-containing protein 1 (BAHD1) is required for overall BAHD1 targeting to chromatin and for optimal repression of the H3K27me3-demarcated genes in mammalian cells. Disruption of direct interaction between BAHD1(BAH) and H3K27me3 by point mutagenesis leads to chromatin remodeling, notably, increased histone acetylation, at its Polycomb gene targets. Mice carrying an H3K27me3-interaction-defective mutation of Bahd1(BAH) causes marked embryonic lethality, showing a requirement of this pathway for normal development. Altogether, this work demonstrates an H3K27me3-initiated signaling cascade that operates through a conserved BAH 'reader' module within BAHD1 in mammals.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据