期刊
NEW PHYTOLOGIST
卷 231, 期 3, 页码 1138-1156出版社
WILEY
DOI: 10.1111/nph.17442
关键词
acetylation; Arabidopsis; HopZ1a; MAP kinases; MKK; plant immunity; Pseudomonas syringae; type III secretion system
资金
- Spanish Ministerio de Ciencia, Innovacion y Universidades [RTI2018-095069-B-I00]
- Spanish Ministerio de Economia y Competitividad [BIO2015-64391-R]
- Programa Operativo Fondos Europeos de Desarrollo Regional (FEDER) Andalucia [UMA18-FEDERJA-070]
- FPU Grant (Predoctoral Fellowship from the Spanish Ministerio de Educacion, Cultura y Deporte [FPU14/04233]
- FEDER
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27040204]
- National Natural Science Foundation of China [31571973]
- China Postdoctoral Science Foundation [2017M621549]
- President's International Fellowship Initiative (PIFI) [2018PB0057, 2020PB0088]
The effector protein HopZ1a from Pseudomonas syringae suppresses plant basal immunity by targeting the Arabidopsis thaliana mitogen-activated protein kinase kinase AtMKK7 through acetylation, leading to inhibition of immune signaling pathways. In vitro and in planta assays demonstrate the interaction between HopZ1a and AtMKK7, with acetylation of a lysine residue crucial for AtMKK7 kinase activity.
The Pseudomonas syringae type III secretion system translocates effector proteins into the host cell cytosol to suppress plant basal immunity. Effector HopZ1a suppresses local and systemic immunity triggered by pathogen-associated molecular patterns (PAMPs) and effectors, through target acetylation. HopZ1a has been shown to target several plant proteins, but none fully substantiates HopZ1a-associated immune suppression. Here, we investigate Arabidopsis thaliana mitogen-activated protein kinase kinases (MKKs) as potential targets, focusing on AtMKK7, a positive regulator of local and systemic immunity. We analyse HopZ1a interference with AtMKK7 by translocation of HopZ1a from bacteria inoculated into Arabidopsis expressing MKK7 from an inducible promoter. Reciprocal phenotypes are analysed on plants expressing a construct quenching MKK7 native expression. We analyse HopZ1a-MKK7 interaction by three independent methods, and the relevance of acetylation by in vitro kinase and in planta functional assays. We demonstrate the AtMKK7 contribution to immune signalling showing MKK7-dependent flg22-induced reactive oxygen species (ROS) burst, MAP kinas (MAPK) activation and callose deposition, plus AvrRpt2-triggered MKK7-dependent signalling. Furthermore, we demonstrate HopZ1a suppression of all MKK7-dependent responses, HopZ1a-MKK7 interaction in planta and HopZ1a acetylation of MKK7 with a lysine required for full kinase activity. We demonstrate that HopZ1a targets AtMKK7 to suppress local and systemic plant immunity.
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