Cytoskeletal control of early mammalian development

The cytoskeleton has been extensively implicated in regulating cell function and behaviour during development. This Review analyses the functional organization of cytoskeletal components in the early mouse embryo, and discusses key roles of the cytoskeleton during early mammalian embryogenesis, including regulation of cell fate specification and morphogenesis of the blastocyst. The cytoskeleton - comprising actin filaments, microtubules and intermediate filaments - serves instructive roles in regulating cell function and behaviour during development. However, a key challenge in cell and developmental biology is to dissect how these different structures function and interact in vivo to build complex tissues, with the ultimate aim to understand these processes in a mammalian organism. The preimplantation mouse embryo has emerged as a primary model system for tackling this challenge. Not only does the mouse embryo share many morphological similarities with the human embryo during its initial stages of life, it also permits the combination of genetic manipulations with live-imaging approaches to study cytoskeletal dynamics directly within an intact embryonic system. These advantages have led to the discovery of novel cytoskeletal structures and mechanisms controlling lineage specification, cell-cell communication and the establishment of the first forms of tissue architecture during development. Here we highlight the diverse organization and functions of each of the three cytoskeletal filaments during the key events that shape the early mammalian embryo, and discuss how they work together to perform key developmental tasks, including cell fate specification and morphogenesis of the blastocyst. Collectively, these findings are unveiling a new picture of how cells in the early embryo dynamically remodel their cytoskeleton with unique spatial and temporal precision to drive developmental processes in the rapidly changing in vivo environment.

Automated summary

The cytoskeleton plays crucial roles in regulating cell function and behavior during early mammalian embryogenesis. The early mouse embryo provides a valuable model for studying cytoskeletal dynamics and interactions in vivo. Through genetic manipulations and live-imaging approaches, researchers are able to uncover novel cytoskeletal structures and mechanisms controlling key developmental processes.