A genetically encoded sensor for measuring serotonin dynamics

Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB(5-HT)) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB(5-HT) detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling. The authors develop a genetically encoded GPCR-based sensor to image serotonin dynamics in behaving animals with high specificity, sensitivity and spatiotemporal resolution.

Automated summary

The study developed a genetically encoded GPCR sensor with high specificity, sensitivity, and subcellular resolution for imaging serotonin dynamics in behaving animals. This technology provides new insights into the dynamics and mechanisms of serotonin signaling.