期刊
NATURE NANOTECHNOLOGY
卷 16, 期 6, 页码 688-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41565-021-00878-4
关键词
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资金
- DTRA [HDTRA1-19-0021]
- NSF [DMREF 1435957, ECCS 1807604, CBET 1805274]
- National Institutes of Health [R21EB024102]
- Office of Naval Research [N0001417WX01318, N0001418WX01042]
- Office of the Under Secretary of Defense for Research and Engineering (USD(RAMP
- E)) through the Applied Research for Advancement of SAMP
- T Priorities (ARAP) Program on Synthetic Biology for Military Environments (SBME)
Living electrodes facilitate signaling within microbial communities, coordinating behavior. This study developed a bioelectronic communication system that allows exchange of information between living cells and an engineered microbial network, enabling real-time control and feedback. The system demonstrates how molecular communication can be propagated to drive biological signal propagation and programmed tasks concurrently.
Living electrodes enable signalling into a microbial community, coordinating behaviour. We developed a bioelectronic communication system that is enabled by a redox signal transduction modality to exchange information between a living cell-embedded bioelectronics interface and an engineered microbial network. A naturally communicating three-member microbial network is 'plugged into' an external electronic system that interrogates and controls biological function in real time. First, electrode-generated redox molecules are programmed to activate gene expression in an engineered population of electrode-attached bacterial cells, effectively creating a living transducer electrode. These cells interpret and translate electronic signals and then transmit this information biologically by producing quorum sensing molecules that are, in turn, interpreted by a planktonic coculture. The propagated molecular communication drives expression and secretion of a therapeutic peptide from one strain and simultaneously enables direct electronic feedback from the second strain, thus enabling real-time electronic verification of biological signal propagation. Overall, we show how this multifunctional bioelectronic platform, termed a BioLAN, reliably facilitates on-demand bioelectronic communication and concurrently performs programmed tasks.
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