Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research

This Review surveys ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS), a highly sensitive, high-throughput technique that is used for analyzing a broad range of metabolites. Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS) variants currently represent the best tools to tackle the challenges of complexity and lack of comprehensive coverage of the metabolome. UHPLC offers flexible and efficient separation coupled with high-sensitivity detection via HRMS, allowing for the detection and identification of a broad range of metabolites. Here we discuss current common strategies for UHPLC-HRMS-based metabolomics, with a focus on expanding metabolome coverage.

Automated summary

This article introduces ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS), a highly sensitive and high-throughput technique for analyzing various metabolites. UHPLC provides efficient separation, while HRMS enables high-sensitivity detection, allowing for the detection and identification of a broad range of metabolites. The common strategies for expanding metabolome coverage using UHPLC-HRMS are also discussed.