期刊
NATURE CELL BIOLOGY
卷 23, 期 5, 页码 526-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41556-021-00672-3
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资金
- US NIH/NCI R01 [CA217648, CA123088, CA099985, CA193136, CA152470, CA216919, CA213566, CA120458]
- NIH through the University of Michigan Rogel Cancer Center Grant [CA46592]
Li et al. identified a lncRNA called LIMIT that regulates MHC-I expression through HSP90 and HSF1, impacting antitumour immune response and immunotherapy efficacy. By activating GBPs and MHC-I, LIMIT enhances tumour immunogenicity and boosts the effectiveness of immunotherapy.
Li et al. identify LIMIT as a lncRNA that modulates MHC-I expression through HSP90 and HSF1, thereby regulating antitumour immune response and the efficacy of immunotherapy. Major histocompatibility complex-I (MHC-I) presents tumour antigens to CD8(+) T cells and triggers anti-tumour immunity. Humans may have 30,000-60,000 long noncoding RNAs (lncRNAs). However, it remains poorly understood whether lncRNAs affect tumour immunity. Here, we identify a lncRNA, lncRNA inducing MHC-I and immunogenicity of tumour (LIMIT), in humans and mice. We found that IFN gamma stimulated LIMIT, LIMIT cis-activated the guanylate-binding protein (GBP) gene cluster and GBPs disrupted the association between HSP90 and heat shock factor-1 (HSF1), thereby resulting in HSF1 activation and transcription of MHC-I machinery, but not PD-L1. RNA-guided CRISPR activation of LIMIT boosted GBPs and MHC-I, and potentiated tumour immunogenicity and checkpoint therapy. Silencing LIMIT, GBPs and/or HSF1 diminished MHC-I, impaired antitumour immunity and blunted immunotherapy efficacy. Clinically, LIMIT, GBP- and HSF1-signalling transcripts and proteins correlated with MHC-I, tumour-infiltrating T cells and checkpoint blockade response in patients with cancer. Together, we demonstrate that LIMIT is a cancer immunogenic lncRNA and the LIMIT-GBP-HSF1 axis may be targetable for cancer immunotherapy.
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