New lanostane-type triterpenoids with proangiogenic activity from the fruiting body of Ganoderma applanatum

Two new lanostane-type triterpenoids, ganoderenicfys A (1) and B (2), together with six related known terpenoids (3-8), were isolated and identified from the fruiting body of Ganoderma applanatum. The structures of these compounds were established on the basis of detailed interpretation of their NMR and HRESIMS data. The absolute configurations of 1 and 2 were determined by quantum chemical electronic circular dichroism (ECD) calculations. All of the isolated compounds were evaluated for their proangiogenic activities in a transgenic fluorescent zebrafish model. Compounds 1-6 displayed dose-dependently proangiogenic activity in a PTK787-induced vascular injury zebrafish model, while compounds 1, 2 and 4 significantly promoted the angiogenesis. This is the first report for proangiogenic activities of lanostane-type triterpenoids.

Automated summary

Two new lanostane-type triterpenoids, ganoderenicfys A (1) and B (2), along with six known related terpenoids, were isolated from Ganoderma applanatum. Their structures were determined through detailed NMR and HRESIMS analysis, with the absolute configurations of 1 and 2 confirmed through quantum chemical ECD calculations. These compounds were found to exhibit proangiogenic activities in a zebrafish model, with compounds 1, 2, and 4 showing significant promotion of angiogenesis, marking the first report of such activity for lanostane-type triterpenoids.