期刊
NANO LETTERS
卷 21, 期 7, 页码 3295-3301出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.1c00725
关键词
Single-molecule FRET; structural biology; DNA nanotechnology; DNA-PAINT; single-molecule multiplexing
类别
资金
- Vrije Programma (SMPS) of the Foundation for Fundamental Research on Matter and Human Frontier Science Program [RGP0026/2019]
The research team introduced a new method that allows for determining multiple distances between FRET pairs in a single object by resolving the FRET efficiency of multiple fluorophore pairs through transient binding of short DNA strands. This FRET X technology is expected to be a tool for high-resolution analysis of biomolecules and nanostructures.
Single-molecule FRET is a versatile tool to study nucleic acids and proteins at the nanometer scale. However, currently, only a couple of FRET pairs can be reliably measured on a single object, which makes it difficult to apply single-molecule FRET for structural analysis of biomolecules. Here, we present an approach that allows for the determination of multiple distances between FRET pairs in a single object. We use programmable, transient binding between short DNA strands to resolve the FRET efficiency of multiple fluorophore pairs. By allowing only a single FRET pair to be formed at a time, we can determine the pair distance with subnanometer precision. The distance between other pairs are determined by sequentially exchanging DNA strands. We name this multiplexing approach FRET X for FRET via DNA eXchange. Our FRET X technology will be a tool for the high-resolution analysis of biomolecules and nanostructures.
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