期刊
NANO LETTERS
卷 21, 期 8, 页码 3393-3400出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.0c04864
关键词
Single particle tracking; Rotation tracking; Nitrogen vacancy centers in nanodiamond; Optically detected magnetic resonance; Cell membrane
类别
资金
- RGC/CRF of HKSAR [C4007-19G]
- ANR/RGC Joint Research Project [A-CUHK404/18]
- EU
- ERC
- Max Planck Society
The research team developed a new technique for tracking both three-dimensional translation and rotation of single diamond particles, which was successfully applied to measure dynamics inside cells and provided important insights into cell metabolic activities.
Correlated translation-orientation tracking of single particles can provide important information for understanding the dynamics of live systems and their interaction with the probes. However, full six-dimensional (6D) motion tracking has yet to be achieved. Here, we developed synchronized 3D translation and 3D rotation tracking of single diamond particles based on nitrogen-vacancy center sensing. We first performed 6D tracking of diamond particles attached to a giant plasma membrane vesicle to demonstrate the method. Quantitative analysis of diamond particles' motion allowed elimination of the geometric effect and revealed the net rotation on the vesicle. 6D tracking was then applied to measure live cell dynamics. Motion characteristics of nanodiamonds on cell membranes under various controlled physiological conditions suggest that the nanodiamonds' rotation is associated with cell metabolic activities. Our technique extends the toolbox of single particle tracking and provides a unique solution to problems where correlated analysis of translation and rotation is critical.
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