期刊
MOLECULES
卷 26, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/molecules26071892
关键词
Bacillus subtilis; NRPS; PKS; amicoumacins; heterologous expression; bioactivities
资金
- National Key R&D Program of China [2019YFA0905700]
- China Postdoctoral Science Foundation [2019M661767]
- National Natural Science Foundation of China [31571887]
- Jiangsu Planned Projects for Postdoctoral Research Funds [2019K015]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Research and Practice Innovation Program for College Graduates of Jiangsu Province [KYCX20_2888]
Bacillus subtilis fmb60, isolated from plant straw compost, exhibits broad-spectrum antimicrobial activities and produces multiple secondary metabolites, including lipoamides and amicoumacin derivatives. These compounds show strong antibacterial activities against harmful foodborne bacteria, indicating potential biocontrol applications. Genomic mining reveals a greater biosynthetic potential for novel natural products production.
Bacillus subtilis fmb60, which has broad-spectrum antimicrobial activities, was isolated from plant straw compost. A hybrid NRPS/PKS cluster was screened from the genome. Sixteen secondary metabolites produced by the gene cluster were isolated and identified using LC-HRMS and NMR. Three lipoamides D-F (1-3) and two amicoumacin derivatives, amicoumacins D, E (4, 5), were identified, and are reported here for the first time. Lipoamides D-F exhibited strong antibacterial activities against harmful foodborne bacteria, with the MIC ranging from 6.25 to 25 mu g/mL. Amicoumacin E scavenged 38.8% of ABTS(+) radicals at 1 mg/mL. Direct cloning and heterologous expression of the NRPS/PKS and ace gene cluster identified its importance for the biosynthesis of amicoumacins. This study demonstrated that there is a high potential for biocontrol utilization of B. subtilis fmb60, and genome mining for clusters of secondary metabolites of B. subtilis fmb60 has revealed a greater biosynthetic potential for the production of novel natural products than previously anticipated.
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