期刊
MOLECULES
卷 26, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/molecules26061578
关键词
desymmetrization; bioreduction; microbial synthesis; asymmetric; microorganism; α -hydroxy ketones
资金
- Nicolaus Copernicus University, Collegium Medicum [166]
This study utilized the bioreduction capabilities of Aureobasidium pullulans strains DSM 14940 and DSM 14941 to successfully synthesize high enantiomeric purity of (S)-benzoin. Mild conditions were employed for benzoin synthesis using two different methodologies, and enrichment of (R)-benzoin was achieved through changes in reaction conditions and additives.
In this study, we examined the Aureobasidium pullulans strains DSM 14940 and DSM 14941 included in the Blossom Protect (TM) agent to be used in the bioreduction reaction of a symmetrical dicarbonyl compound. Both chiral 2-hydroxy-1,2-diphenylethanone antipodes were obtained with a high enantiomeric purity. Mild conditions (phosphate buffer [pH 7.0, 7.2], 30 degrees C) were successfully employed in the synthesis of (S)-benzoin using two different methodologies: benzyl desymmetrization and rac-benzoin deracemization. Bioreduction carried out with higher reagent concentrations, lower pH values and prolonged reaction time, and in the presence of additives, enabled enrichment of the reaction mixture with (R)-benzoin. The described procedure is a potentially useful tool in the synthesis of chiral building blocks with a defined configuration in a simple and economical process with a lower environmental impact, enabling one-pot biotransformation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据