4.6 Article

Non-canonical metabolic pathways in the malaria parasite detected by isotope-tracing metabolomics

期刊

MOLECULAR SYSTEMS BIOLOGY
卷 17, 期 4, 页码 -

出版社

WILEY
DOI: 10.15252/msb.202010023

关键词

haloacid dehalogenase; mass spectrometry; metabolite repair; Plasmodium; SHMT

资金

  1. University of Melbourne
  2. NHMRC [APP1098992]
  3. Australian Research Council [DP180102729]
  4. National Institute of Health [2R01AI103280-06]
  5. Bioplatforms Australia

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This study used multiplex C-13-labelling coupled with untargeted mass spectrometry to generate a draft metabolome of P. falciparum and its host erythrocyte. It identified previously uncharacterized enzymes crucial for parasite development and proliferation, as well as highlighted the activity of metabolite repair pathways in rapidly growing parasite stages, suggesting new opportunities for drug discovery.
The malaria parasite, Plasmodium falciparum, proliferates rapidly in human erythrocytes by actively scavenging multiple carbon sources and essential nutrients from its host cell. However, a global overview of the metabolic capacity of intraerythrocytic stages is missing. Using multiplex C-13-labelling coupled with untargeted mass spectrometry and unsupervised isotopologue grouping, we have generated a draft metabolome of P. falciparum and its host erythrocyte consisting of 911 and 577 metabolites, respectively, corresponding to 41% of metabolites and over 70% of the metabolic reaction predicted from the parasite genome. An additional 89 metabolites and 92 reactions were identified that were not predicted from genomic reconstructions, with the largest group being associated with metabolite damage-repair systems. Validation of the draft metabolome revealed four previously uncharacterised enzymes which impact isoprenoid biosynthesis, lipid homeostasis and mitochondrial metabolism and are necessary for parasite development and proliferation. This study defines the metabolic fate of multiple carbon sources in P. falciparum, and highlights the activity of metabolite repair pathways in these rapidly growing parasite stages, opening new avenues for drug discovery.

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