期刊
MOLECULAR PLANT PATHOLOGY
卷 22, 期 6, 页码 683-693出版社
WILEY
DOI: 10.1111/mpp.13055
关键词
chitin; effector; LysM; virulence; Zymoseptoria
资金
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek
- Biotechnology and Biological Sciences Research Council [BB/P016855/1]
- Deutsche Forschungsgemeinschaft [EXC 2048/1, 390686111]
- China Scholarship Council
Chitin is a major component of fungal cell walls and triggers immune responses in plants. The wheat pathogen Zymoseptoria tritici secretes LysM effector proteins to avoid host immune activation. Three LysM effectors, including Mgx1LysM, play roles in disarming chitin-triggered wheat immunity with varying degrees of contribution to the virulence of Z. tritici.
Chitin is a major structural component of fungal cell walls and acts as a microbe-associated molecular pattern (MAMP) that, on recognition by a plant host, triggers the activation of immune responses. To avoid the activation of these responses, the Septoria tritici blotch (STB) pathogen of wheat, Zymoseptoria tritici, secretes LysM effector proteins. Previously, the LysM effectors Mg1LysM and Mg3LysM were shown to protect fungal hyphae against host chitinases. Furthermore, Mg3LysM, but not Mg1LysM, was shown to suppress chitin-induced reactive oxygen species (ROS) production. Whereas initially a third LysM effector gene was disregarded as a presumed pseudogene, we now provide functional data to show that this gene also encodes a LysM effector, named Mgx1LysM, that is functional during wheat colonization. While Mg3LysM confers a major contribution to Z. tritici virulence, Mgx1LysM and Mg1LysM contribute to Z. tritici virulence with smaller effects. All three LysM effectors display partial functional redundancy. We furthermore demonstrate that Mgx1LysM binds chitin, suppresses the chitin-induced ROS burst, and is able to protect fungal hyphae against chitinase hydrolysis. Finally, we demonstrate that Mgx1LysM is able to undergo chitin-induced polymerization. Collectively, our data show that Z. tritici utilizes three LysM effectors to disarm chitin-triggered wheat immunity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据