期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 65, 期 10, 页码 -出版社
WILEY
DOI: 10.1002/mnfr.202000845
关键词
exosomes; LC-MS/MS; lipids; necrotizing enterocolitis; neonate
资金
- National Natural Science Foundation of China [81971427]
- Jiangsu Province Women and Children Health Key Talents [FRC201740]
- National Natural Science Foundation for Youth Scholars of China [81901512]
- Nanjing Medical Science and Technique Development Foundation for Distinguished Young Scholars [JQX18010]
- Science and Technology Development Fund of Nanjing Medical University [NMUB2018127]
The study reveals the complexity of lipid expression profiles in exosomes derived from preterm and term human milk. The top 50 lipids identified in the exosomes regulate intestinal epithelial cell function via the ERK/MAPK pathway, providing novel mechanistic insight on how human milk prevents the development of necrotizing enterocolitis (NEC).
Scope: Human milk can prevent the development of necrotizing enterocolitis (NEC). Human milk is rich in cargo-carrying exosomes that participate in intercellular communication. This study investigated the effects of term and preterm human milk-derived exosomes, and elucidated their lipid expression profiles. Methods and Results: Milk from healthy mothers is collected who have delivered full-term or preterm infants, and exosomes are isolated and quantified. Administration of term and preterm milk exosomes significantly enhances epithelial proliferation and migration in vitro, and ameliorates the severity of NEC in vivo. A total of 395 lipids are identified in term and preterm human milk-derived exosomes. Bioinformatics analysis and western blotting reveal that top 50 lipids regulate intestinal epithelial cell function via the Extracellular-Signal-Regulated Kinase/Mitogen Activated Protein Kinase (ERK/MAPK) pathway. Conclusion: This study reveals for the first time the lipidomic complexities in exosomes derived from preterm and term milk. The results provide novel mechanistic insight on how human milk prevents the development of NEC.
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