期刊
MOLECULAR CANCER RESEARCH
卷 19, 期 7, 页码 1196-1207出版社
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/1541-7786.MCR-20-0413
关键词
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资金
- Department of Pharmaceutical Sciences at the University of the Sciences
- Department of Biomedical Sciences at Cooper Medical School of Rowan University
- New Jersey Commission on Cancer Research
- W.W. Smith Charitable Trust
- National Institute of Arthritis, and Musculoskeletal and Skin Diseases [R15AR069935]
- Rowan University under the Camden Health Research Initiative
Nuclear envelope proteins, specifically emerin, play a crucial role in regulating nuclear size and structure in cancer contexts. Alterations in emerin expression impact nuclear size and cancer cell migration, ultimately affecting metastasis. Modulating emerin expression and function presents novel therapeutic targets for combating metastasis, as increased emerin expression can rescue cancer metastasis.
Nuclear envelope proteins play an important role in regulating nuclear size and structure in cancer. Altered expression of nuclear lamins are found in many cancers and its expression is correlated with better clinical outcomes. The nucleus is the largest organelle in the cell with a diameter between 10 and 20 mu m. Nuclear size significantly impacts cell migration. Nuclear structural changes are predicted to impact cancer metastasis by regulating cancer cell migration. Here we show emerin regulates nuclear structure in invasive breast cancer cells to impact cancer metastasis. Invasive breast cancer cells had 40% to 50% less emerin than control cells, which resulted in decreased nuclear size. Overexpression of GFP-emerin in invasive breast cancer cells rescued nuclear size and inhibited migration through 3.0 and 8.0 mu m pores. Mutational analysis showed emerin binding to nudeoskeletal proteins was important for its regulation of nuclear structure, migration, and invasion. Importantly, emerin expression inhibited lung metastasis by 91% in orthotopic mouse models of breast cancer. Emerin nucleoskeleton-binding mutants failed to inhibit metastasis. These results support a model whereby emerin binding to the nucleoskeleton regulates nuclear structure to impact metastasis. In this model, emerin plays a central role in metastatic transformation, because decreased emerin expression during transformation causes the nudear structural defects required for increased cell migration, intravasation, and extravasation. Implications: Modulating emerin expression and function represents new targets for therapeutic interventions of metastasis, because increased emerin expression rescued cancer metastasis.
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