Radiomics Biomarkers Correlate with CD8 Expression and Predict Immune Signatures in Melanoma Patients

Treatment for metastatic melanoma includes targeted and/or immunotherapy. Although many patients respond, only a subset has complete response. As late-stage patients often have multiple tumors in difficult access sites, non-invasive techniques are necessary for the development of predictive/prognostic biomarkers. PET/CT scans from 52 patients with stage III/IV melanoma were assessed and CT image parameters were evaluated as prognostic biomarkers. Analysis indicated patients with high standard deviation or high mean of positive pixels (MPP) had worse progression-free survival (P = 0.00047 and P = 0.0014, respectively) and worse overall survival (P = 0.0223 and P = 0.0465, respectively). Whole-exome sequencing showed high MPP was associated with BRAF mutation status (P = 0.0389). RNA-sequencing indicated patients with immune cold signatures had worse survival, which was associated with CT biomarker, MPP4 (P = 0.0284). Multiplex immunofluorescence confirmed a correlation between CD8 expression and image biomarkers (P = 0.0028).

Automated summary

In the treatment of metastatic melanoma, high standard deviation or high mean of positive pixels in CT imaging were found to be associated with worse prognosis. In addition, high MPP was linked to BRAF mutation status. Immune cold signatures and CT biomarker MPP4 were also found to impact patient survival. Immunofluorescence confirmed a correlation between CD8 expression and image biomarkers.