期刊
MICROCHIMICA ACTA
卷 188, 期 4, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-021-04788-z
关键词
DNA biosensor; Gold nanorods; Lateral flow; Visual detection
资金
- Natural Science Foundation of Anhui province [1808085QH264, 1908085MB54]
- Key Research and Development Projects of Anhui Province [202004a07020018]
- Major project of Anhui Provincial Department of Education [KJ2019ZD58]
- Wanjiang Scholar Award of Anhui Province
- National Natural Science Foundation of China [31700735, 21890740, 21890742, 21727815]
- Projects of Anhui Science and Technology University for Talent introduction [SKYJ201903]
A gold nanorod-based lateral flow nucleic acid biosensor was developed for visual detection of DNA with high sensitivity and short test time. By forming a sandwich-type complex on the LFNAB, target DNA could be detected without instrumentation, and quantitative analysis was achieved using a portable strip reader.
A gold nanorod (AuNR)-based lateral flow nucleic acid biosensor (LFNAB) is reported for visual detection of DNA with a short test time and high sensitivity. AuNRs with an approximate length of 60 nm were utilized as a colored tag to label the detection DNA probe (Det-DNA). The capture DNA probe (Cap-DNA) was immobilized on the test region of LFNAB. Sandwich-type complex was formed among the AuNR-Det-DNA, target DNA (Tar-DNA), and Cap-DNA on the LFNAB by Watson-Crick base pairing. In the presence of Tar-DNA, AuNRs were thus seized on the test region of LFNAB, and the accumulation of AuNRs subsequently produced a characteristic colored band. The optimized LFNAB was able to detect 10 pM Tar-DNA without instrumentation. Quantitative analysis could be established by measuring the intensity of test band using a portable strip reader, and the detection limit of 2 pM target DNA was achieved on the LFNAB without signal amplification. The detection limit of the AuNR-based LFNAB is 250-fold lower than that of gold nanoparticle (AuNP)-based LFNABs. This work unveiled a sensitive, rapid, and economical strategy for the detection of nucleic acids, and simultaneously opening new promising routes for disease diagnosis and clinical applications.S
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