A self-correcting fluorescent assay of tyrosinase based on Fe-MIL-88B-NH2 nanozyme

A self-correcting fluorescent assay of tyrosinase (TYR) was developed by utilization of Fe-MIL-88B-NH2 as a peroxidase-like nanozyme and a capture probe. Fe-MIL-88B-NH2 nanozyme was selected as an electron donor, and the oxidization product (dopamine-o-quinone) acts as an energy acceptor. First, TYR catalyzes the oxidation of tyramine hydrochloride to dopamine and then to dopamine-o-quinone. Second, Fe-MIL-88B-NH2 with intrinsic peroxidase-like activity decomposes H2O2 to produce .OH radicals, which further accelerate the oxidation of dopamine to dopamine-o-quinone. Excessive H2O2 and .OH radicals reduce the interferences from ascorbic acid at the same time providing a self-correcting ability. Dopamine-o-quinone reacts with -NH2 groups on the ligand of Fe-MIL-88B-NH2 through Michael reaction which results in fluorescence quenching. Under 365-nm excitation, the fluorescence emission intensity at 452 nm gradually decreased with increasing TYR concentration varying from 0 to 10 U mL(-1). The linear range is from 1 to 5 U mL(-1) and the detection limit is 0.05679 U mL(-1). This self-correcting fluorescent assay of tyrosinase exhibits good sensitivity and selectivity which is also successfully applied for tyrosinase inhibitor detection.

Automated summary

An innovative self-correcting fluorescent assay of tyrosinase was developed using Fe-MIL-88B-NH2 as a peroxidase-like nanozyme and capture probe. This method exhibited good sensitivity and selectivity, successfully applied in detecting tyrosinase inhibitors.