Rapid and sensitive determination of Pseudomonas aeruginosa by using a glassy carbon electrode modified with gold nanoparticles and aptamer-imprinted polydopamine

Pseudomonas aeruginosa causes a long-term chronic disease that low concentrations of Pseudomonas aeruginosa can cause infection; therefore, rapid detection of this Pseudomonas aeruginosa is very important. This study presents a method with a combination of molecular imprinting and aptasensing in order to measure Pseudomonas aeruginosa (P. aeruginosa). The double exact molecular recognition property of the molecular imprinting polymers and aptamers led to superb sensing properties. In this study, gold nanoparticles were used as specific intermediate and interface in order to increase the aptamer sequence loading rate. The capability of this method was investigated by several electrochemical techniques. The sensor renders a wide linear dynamic concentration range of 101 to107 CFU center dot ml-1 with low detection limit of 1 CFU center dot ml-1. In addition, satisfactory results were found in determining P. aeruginosa using blood samples. This sensor has many advantages, such as low cost, high stability, low response time, and high sensitivity for P. aeruginosa measurement.

Automated summary

The study introduces a method combining molecular imprinting and aptasensing for rapid and accurate detection of Pseudomonas aeruginosa, showing a wide linear dynamic concentration range and low detection limit. The sensor has advantages of low cost, high stability, low response time, and high sensitivity for measuring P. aeruginosa.