期刊
MICROBIAL CELL FACTORIES
卷 20, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12934-021-01592-5
关键词
Staphylococcus aureus; Osteomyelitis; Antibiotic tolerance; Bacillus subtilis; Membrane permeabilization; Biofilm
资金
- National Natural Science Foundation of China [81772366, 82072459]
- Major Program of National Natural Science Foundation of China [81830079]
- Science and Technology Key Planning Project of Guangdong [2019B020201013]
- Natural Science Foundation of Guangdong Province, China [2021A1515010773]
Cell-free supernatant of Bacillus subtilis (B. subtilis CFS) can effectively kill Staphylococcus aureus, increase its susceptibility to penicillin and gentamicin, suppress gene expression, alter membrane components, and enhance membrane permeabilization. This suggests that B. subtilis CFS may be a potential resistance-modifying agent against beta-lactam antibiotics for S. aureus.
As treatment of Staphylococcus aureus (S. aureus) osteomyelitis is often hindered by the development of antibiotic tolerance, novel antibacterial therapeutics are required. Here we found that the cell-free supernatant of Bacillus subtilis (B. subtilis CFS) killed planktonic and biofilm S. aureus, and increased S. aureus susceptibility to penicillin and gentamicin as well. Further study showed that B. subtilis CFS suppressed the expression of the genes involved in adhesive molecules (Cna and ClfA), virulence factor Hla, quorum sensing (argA, argB and RNAIII) and biofilm formation (Ica and sarA) in S. aureus. Additionally, our data showed that B. subtilis CFS changed the membrane components and increased membrane permeabilization of S. aureus. Finally, we demonstrated that B. subtilis CFS increased considerably the susceptibility of S. aureus to penicillin and effectively reduced S. aureus burdens in a mouse model of implant-associated osteomyelitis. These findings support that B. subtilis CFS may be a potential resistance-modifying agent for beta-lactam antibiotics against S. aureus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据