期刊
出版社
ELSEVIER
DOI: 10.1016/j.msec.2021.111984
关键词
LDE nanoemulsion; Photodynamic therapy; Phthalocyanine; Glioblastoma
资金
- Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES)
- State of Sao Paulo Research Foundation (FAPESP) [2013/50181-1, FINEP 01.10.0758.01]
- National Council for Scientific and Technological Development (CNPq) [25000.077093/2015-86]
- FAPESP [2013/50181-1, 2014/11870-9, 2012/25216-3, 2018/10237-1, 2017/16356-0]
- National Institute of Science and Technology (INCT) Nanobiotechnology project [573880/2008-5]
- project PRONON-SIPAR [25000.077093/2015-86]
LDE, a cholesterol-rich nanoemulsion, showed high encapsulation rate and stability, making it an effective drug delivery system. LDEPc exhibited higher light activity and promoted apoptosis in U-87 MG cells, supporting its use as a new theranostic system.
We have prepared and characterized a cholesterol-rich nanoemulsion called LDE, a mimic of classic lipoprotein macromolecules, that can be applied as a new drug delivery system for aluminum phthalocyanine chloride (PcAlCl). The LDE containing PcAlCl system prepared herein had mean size and zeta potential of 127 nm and -29 mV, respectively, and encapsulation rate efficiency was 81%, and stability of 17 months. Compared to classical liposomes, LDE was more efficient, especially in brain diseases like glioblastoma (GBM), as revealed by tests on the U-87 MG cell line. The LDEPc formulation did not display dark cytotoxicity, as expected. The best light dose for LDEPc was 1.0 J?cm? 2: its activity was 55% higher than PcAlCl in a compatible organic medium. In the U-87 MG cells, apoptosis was the preferential pathway activated by PDT. These results strongly support the use of LDE as a new theranostic system.
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